Choose the CORRECT Order of Procedure in Performing Chromatin Immunoprecipitation

question 54

Multiple Choice

Choose the CORRECT order of procedure in performing chromatin immunoprecipitation (ChIP) . 1. DNA sequencing and fragment identification
2) Removal of crosslinking and separation of DNA and proteins
3) Crosslinking proteins and chromosomes via UV
4) Antibody incubation for immunoprecipitation
5) Protein degradation via proteases
6) Cell lysis and chromosome fragmentation

• A) 1, 3, 2, 4, 5, 6
• B) 2, 6, 5, 1, 3, 4
• C) 4, 3, 2, 5, 1, 5
• D) 3, 6, 4, 2, 5, 1
• E) 5, 1, 3, 6, 4, 2

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Definitions:

Estimated Dose

The approximate amount of a medication or treatment calculated to have a desired therapeutic effect in the absence of exact measurement.

After Meals

A term used to indicate that medication should be taken or an action performed after eating.

Without

Lacking or not including something; absence of.

Immediately

Without delay; happening right away.