An E Coli Strain of Chromosomal Genotype LacI^- LacP⁺ lacO⁺ LacZ⁺

question 65

Essay

An E. coli strain of chromosomal genotype lacI^- lacP⁺ lacO⁺ lacZ⁺ lacY⁺ constitutively expresses the genes of the lac operon. The strain is converted to wild-type lac operon regulation by the addition of an extra piece of DNA. What gene or genes are contained on this extra DNA that explain this conversion to wild type? Include an explanation of cis- or trans-acting factors and how they work.

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Definitions:

Deadweight Loss

The falloff in economic efficiency that strikes when equilibrium for a good or service isn’t met or is beyond reach.

Tax Revenue

The revenue governments receive as a consequence of taxes.

Deadweight Loss

Failure to reach free market equilibrium for a good or service leads to a loss in economic efficiency.

Labor Supply

The total hours that workers are willing and able to work at a given rate of pay, across different job markets or sectors.