Imagine the Following Scenario: You Take the Regulatory Region of the Trp

question 24

Essay

Imagine the following scenario: You take the regulatory region of the trp operon (including the promoter, operator, and 5' UTR) and attach it upstream of the structural genes of the lac operon. You then introduce this artificial construct into a mutant strain in which its own lac operon is completely nonfunctional. Indicate the level of ß-galactosidase activity in each of the following cases and explain why you expect that level of activity:
a. No tryptophan, no lactose
b. High tryptophan, high lactose

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Definitions:

Filtration Slits

Narrow gaps between the podocytes in the glomerulus of the kidney through which filtration of blood plasma occurs.

Podocytes

Highly specialized cells in the kidney that wrap around capillaries of the glomerulus, involved in filtration of blood to form urine.

Renal Corpuscles

The initial filtering component of a nephron in the kidney, consisting of a glomerulus and Bowman's capsule.