Use the Following to Answer Questions
an Auxotrophic E

question 38

Multiple Choice

Use the following to answer questions
An auxotrophic E. coli strain requires adenine to grow because of a mutation in a gene for an adenine synthesis enzyme. The following shows part of the wild-type and mutant alleles of the gene, including the start codon. The bottom strand is the template for transcription. $$\begin{array} { c c } \text { ade } 1 ^ { + } \text {wild-type allele } & \text { ade1 } ^ { - } \text {mutant allele } \\\text { 5'...TTATGGGCAAGATCCCA...3' } & \text { 5'....TTA TGGGCTAGATCCCA...3' } \\\text { 3'...AATACCCGTTCTAGGGT...5' } & \text { 3'...AATATGGGCTAGATCCCA...3' }\end{array}$$
-How does this mutation change the mRNA?

• A) The third codon in the mRNA is changed from AAG (lysine) to UAG (stop).
• B) The third codon in the mRNA is changed from GCA (alanine) to GCU (alanine).
• C) The third codon in the mRNA is changed from CAA (glutamine) to CUA (leucine).
• D) The third codon in the mRNA is changed from CGU (arginine) to CGA (arginine).
• E) There is no change in the mRNA.

---

Definitions:

Racial Segregation

The enforced separation of different racial groups within a country, community, or establishment, often resulting in discriminatory practices.

Civil War Amendments

Refers to the 13th, 14th, and 15th amendments to the United States Constitution, which abolished slavery, granted citizenship rights, and protected voting rights regardless of race, respectively.

Due Process

A fundamental principle in the justice system that guarantees fair treatment through the normal judicial system, especially a citizen's entitlement to notice of a charge and a hearing before any governmental deprivation of life, liberty, or property.

Plessy v. Ferguson

A landmark 1896 U.S. Supreme Court case that upheld the constitutionality of racial segregation under the "separate but equal" doctrine.