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an Auxotrophic E

question 38

Multiple Choice

Use the following to answer questions
An auxotrophic E. coli strain requires adenine to grow because of a mutation in a gene for an adenine synthesis enzyme. The following shows part of the wild-type and mutant alleles of the gene, including the start codon. The bottom strand is the template for transcription. $$\begin{array} { c c } \text { ade } 1 ^ { + } \text {wild-type allele } & \text { ade1 } ^ { - } \text {mutant allele } \\\text { 5'...TTATGGGCAAGATCCCA...3' } & \text { 5'....TTA TGGGCTAGATCCCA...3' } \\\text { 3'...AATACCCGTTCTAGGGT...5' } & \text { 3'...AATATGGGCTAGATCCCA...3' }\end{array}$$
-How does this mutation change the mRNA?

• A) The third codon in the mRNA is changed from AAG (lysine) to UAG (stop).
• B) The third codon in the mRNA is changed from GCA (alanine) to GCU (alanine).
• C) The third codon in the mRNA is changed from CAA (glutamine) to CUA (leucine).
• D) The third codon in the mRNA is changed from CGU (arginine) to CGA (arginine).
• E) There is no change in the mRNA.

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