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What Would You Add to an in Vitro Transcription System

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What would you add to an in vitro transcription system that contains an E. coli gene for glyceraldehyde 3-phosphate dehydrogenase, an enzyme in glycolysis, in order to get transcription that begins from the normal transcription start site?


Definitions:

Secondary Control

A coping mechanism that involves adjusting one's own goals and efforts to deal with situations that are perceived as uncontrollable.

Primary Control

The effort to change the external world so that it aligns with one's personal needs, desires, or goals.

Modify Reality

The act of altering the perception or understanding of the real world, often through technology, art, or psychological means.

Physical Exercise

Any bodily activity that enhances or maintains physical fitness and overall health and wellness, often performed for various reasons including strengthening muscles and the cardiovascular system.

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