Your Goal Is to Achieve a High Level of Transcription-A

question 36

Essay

Your goal is to achieve a high level of transcription-a level similar to its in vivo levels-of a eukaryotic gene in an in vitro transcription system. You add the DNA template for this gene, which contains the following, to the in vitro transcription system: a TATA box sequence located -25 to -30 bp upstream of the transcription start site, the sequence for the gene, and terminator sequences. How would you interpret the result if you do not get high levels of RNA transcription, and what corrective measures would you take to achieve your goal?

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Definitions:

Agnosia

A neurological disorder marked by the inability to recognize objects, persons, sounds, shapes, or smells while the specific sense is not defective nor is there any significant memory loss.

Distal Stimulus

Objects or events in the external world that provide the physical energy that is then converted by the sensory organs into neural energy, leading to perception.

Occipital Stimulus

A stimulus that targets the occipital lobe of the brain, primarily involved in visual processing.

Proximal Stimulus

The physical energy from a stimulus that directly interacts with sensory receptors.