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Explain the Effect on DNA Replication of Mutations That Destroy

question 9

Essay

Explain the effect on DNA replication of mutations that destroy each of the following activities in DNA polymerase. Also, for each kind of mutation, how might you detect the effect in an in vitro replication reaction?
a. 5'to 3' polymerase
b. 5'to 3' exonuclease
c. 3' to 5' exonuclease


Definitions:

Honest Error

A mistake made without malicious intent or knowledge of its falsehood, often considered in legal and ethical considerations.

Underestimating Cost

The act of calculating or predicting a cost to be lower than it eventually turns out to be.

Personally Liable

The legal responsibility of an individual to fulfill a debt or obligation from their own assets.

Partnership Debt

Financial obligations for which all partners in a partnership are jointly and severally liable.

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