A Researcher Inserted DNA Fragments from an Organism into Plasmids

question 64

Multiple Choice

A researcher inserted DNA fragments from an organism into plasmids and introduced the modified plasmids into bacterial cells. Which of the following methods would be the most efficient means of identifying which clones contain a specific gene of interest?

• A) use a microarray to detect a transcript of the gene
• B) use a labeled synthetic probe complementary to the gene sequence
• C) use electrophoresis to identify plasmids containing an insert of the expected size
• D) sequence the DNA of the plasmids from each isolate
• E) assay for activity of the gene product

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Definitions:

Information Deficit

A lack or shortfall of necessary information or knowledge in a particular area.

Coleaders

Two or more individuals who share the leadership responsibilities in a group setting, such as in therapy groups or team projects.

Leadership Style

The manner and approach of providing direction, implementing plans, and motivating people.

Countertransference

The phenomenon where a therapist projects their own unresolved feelings onto the client.