When FISH Protocols Call for the Use of "Aged" Slides

question 22

Multiple Choice

When FISH protocols call for the use of "aged" slides of cells, how can this be accomplished?

• A)Baking slides at 60° C for 4-16 hours.
• B)Soaking slides in pH 7.0 TRIS buffer and 1% ethanol.
• C)Washing slides in pH 6.5 normal saline followed by 1% methanol.
• D)Freezing slides at - 70° C for 1 week prior to analysis.

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Definitions:

Forgets

Refers to the inability to retrieve information from memory, which can be temporary or permanent, depending on the context and individual.

Epigenetics

The analysis of inheritable modifications in the way genes are expressed, without any changes occurring to the actual DNA sequence.

Molecular Mechanisms

A term referring to the specific biochemical interactions that occur within living cells to maintain and regulate their function and response.

Environmental Influence

The effect that surroundings, including cultural and natural factors, have on the development and behavior of individuals.