The Three Basic Steps of Polymerase Chain Reaction (PCR)are Denaturation

question 44

Multiple Choice

The three basic steps of polymerase chain reaction (PCR) are denaturation, primer annealing, and primer extension. Which specification listed below is correct?

• A)Primer design-oligonucleotide of 15- 30 base pairs
• B)Primer extension-10 minutes during each cycle
• C)Primer annealing-95° C for 1 minute
• D)Denaturation-50° C for 5 minutes
• E)Primer extension-37° C when Taq polymerase is used

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Definitions:

P-Value

The probability that the observed data would occur if the null hypothesis were true, used as a measure to decide whether to reject the null hypothesis.

Test Statistic

A numerical figure derived from sample data for the purpose of testing a hypothesis within the field of statistics.

Randomized Block

A design in experiments where the experimental subjects are first divided into blocks based on external factors that might influence the results, and then randomly assign treatments within each block to minimize variability.

Analysis of Variance

A statistical method used to test differences between two or more means by analyzing variance among and between groups.