If the Optimal PH for Taq Polymerase Is About 7

question 4

Multiple Choice

If the optimal pH for Taq polymerase is about 7.2, why are the reaction buffers manufactured to have a pH of about 8.5?

• A)Unincorporated nucleotides are unstable at neutral to acidic pHs.
• B)Hydrogen bonds form poorly at neutral to acidic pHs.
• C)Optimal reaction conditions for Taq polymerase are generally not used because rapid incorporation of nucleotides into a growing DNA chains leads to more errors.
• D)pH is temperature dependent, and at the optimal temperature for Taq polymerase to function, the buffer pH will decrease.

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