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-A PCR Amplification Was Done on a Positive Control Containing

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  -A PCR amplification was done on a positive control containing the template DNA, a negative control without the template DNA and two samples. Oligonucleotide primers of 21 base pairs were used, and the expected amplicon product is about 160 base pairs. Observe the agarose gel electrophoresis done on the amplicons, and give the most appropriate interpretations.   A) The assay must be repeated because the positive control has more than one amplicon. B) The assay must be repeated because the amplicon is not the right size. C) The assay must be repeated because there is evidence of  primer dimer  formation. D) Sample 1 is positive for the template DNA, and sample 2 is negative for the template DNA. E) The assay must be repeated because the negative control is not negative.
-A PCR amplification was done on a positive control containing the template DNA, a negative control without the template DNA and two samples. Oligonucleotide primers of 21 base pairs were used, and the expected amplicon product is about 160 base pairs. Observe the agarose gel electrophoresis done on the amplicons, and give the most appropriate interpretations.
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Definitions:

Standard Deviation

A measure of the amount of variation or dispersion in a set of values, indicating how much the values diverge from the mean.

Normal Distribution

A type of continuous probability distribution for a real-valued random variable, characterized by a bell-shaped curve, symmetrical around its mean.

Variance

A measure of the dispersion indicating how much the numbers in a data set differ from the mean of the set.

Normal Distribution

A symmetric, bell-shaped distribution of data where most of the observations cluster around the central peak and the probabilities for values further away from the mean taper off equally in both directions.

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