Review the PCR Protocol Herein and Identify Its Name

question 2

Multiple Choice

Review the PCR protocol herein and identify its name. A single- stranded probe with internal complementary sequences folds on itself into a "hairpin" shape bringing a fluorescent molecule at one end of the strand in proximity to the quencher molecule at the other end of the strand. After PCR product is formed, during the next denaturation step the probe becomes single- stranded, binds to PCR target DNA, and separates the fluorescent molecule from the quencher molecule. PCR product is detected as increased fluorescence.

• A)Molecular beacon PCR
• B)5' RACE PCR
• C)Reverse transcriptase PCR
• D)Branched chain PCR
• E)TaqMan PCR

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Definitions:

BGM

Blood Glucose Monitoring, a system used to test the concentration of glucose in the blood, crucial for diabetes management.

Hypoglycemic Episode

A condition characterized by a dangerously low level of glucose in the blood, potentially causing symptoms like trembling, sweating, and confusion.

Bedtime Snacks

A small meal or snack eaten before bed, often recommended for managing blood sugar levels in certain conditions.

Intermediate-Acting Insulin

A type of insulin with a start time of 1 to 3 hours, peak time of 5 to 8 hours, and duration of 12 to 16 hours, used to control blood sugar levels in diabetes.