How Does One Assure That All PCR Amplicons Are Double-

question 40

Multiple Choice

How does one assure that all PCR amplicons are double- stranded?

• A)By lengthening the final primer extension step from 1-2 minutes to up to 10 minutes.
• B)By adding DNA ligase to the mixture.
• C)By holding the reaction mixture at annealing temperature for about 5 minutes at the end of the cycle.
• D)By making sure the dNTPs in the mixture are in excess.

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Definitions:

Stereotypes

Widely held but fixed and oversimplified image or idea of a particular type of person or thing.

Positive Assumptions

The concept of maintaining an optimistic outlook and expecting good outcomes, often leading to positive behavior and attitudes towards life and challenges.

Negative Assumptions

Cognitive biases or beliefs that lead an individual to view situations or outcomes more pessimistically, potentially contributing to anxiety and depression.

Cognitive Dissonance

The unease experienced mentally by an individual who possesses two or more contradictory beliefs, ideas, or values at the same time.