-A PCR Amplification Was Done on a Positive Control Containing

question 34

Multiple Choice

-A PCR amplification was done on a positive control containing the template DNA, a negative control without the template DNA and two samples. Oligonucleotide primers of 21 base pairs were used, and the expected amplicon product is about 160 base pairs. Observe the agarose gel electrophoresis done on the amplicons, and give the most appropriate interpretations.
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• A)The assay must be repeated because the positive control has more than one amplicon.
• B)The assay must be repeated because the amplicon is not the right size.
• C)The assay must be repeated because there is evidence of "primer dimer" formation.
• D)Sample 1 is positive for the template DNA, and sample 2 is negative for the template DNA.
• E)The assay must be repeated because the negative control is not negative.

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Definitions:

Cephalic End

The cephalic end refers to the head end of an organism or embryo, where sensory organs and the brain are often located, opposite the caudal or tail end.

Endoderm

The innermost of the three primary germ layers in the embryo, giving rise to the lining of the digestive and respiratory systems.

Digestive Tube

A continuous hollow tube extending from the mouth to the anus, through which food is processed and nutrients absorbed.

Plankton

Free-floating, mainly microscopic aquatic organisms found in the upper layers of the water; consisting of phytoplankton and zooplankton. Compare with nekton.