Review the PCR Protocol Herein and Identify Its Name

question 2

Multiple Choice

Review the PCR protocol herein and identify its name. A single- stranded probe with internal complementary sequences folds on itself into a "hairpin" shape bringing a fluorescent molecule at one end of the strand in proximity to the quencher molecule at the other end of the strand. After PCR product is formed, during the next denaturation step the probe becomes single- stranded, binds to PCR target DNA, and separates the fluorescent molecule from the quencher molecule. PCR product is detected as increased fluorescence.

• A)Molecular beacon PCR
• B)5' RACE PCR
• C)Reverse transcriptase PCR
• D)Branched chain PCR
• E)TaqMan PCR

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Definitions:

Experienced

Having practical knowledge or skill in a particular area through direct involvement or participation.

Androgyny

A gender expression that includes a combination of masculine and feminine characteristics.

Self-Confident

The belief in one's own abilities, qualities, and judgment, reflecting a positive view of oneself.

Competitive

The desire to win or be more successful than others, often involving comparison against a standard or opponent.