Which of the Following Is the Correct Sequence of PCR

question 89

Multiple Choice

Which of the following is the correct sequence of PCR?

• A) Heat to 70°C (to separate DNA), then cool to 50°C (to bind primers and enzymes), and then reheat to 90°C (to synthesize DNA).
• B) Heat to 90°C (to separate DNA), then cool to 50°C (to bind primers and enzymes), and then reheat to 70°C (to synthesize DNA).
• C) Heat to 70°C (to bind primers and enzymes), then cool to 50°C (to synthesize DNA), and then reheat to 90°C (to separate DNA).
• D) Heat to 90°C (to bind primers and enzymes), then cool to 50°C (to separate DNA), and then reheat to 70°C (to synthesize DNA).

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Definitions:

Similar

Resembling without being identical; having qualities in common or a close resemblance.

Same

Identical; exactly alike or equal in some specified quality or characteristic.

Likeness

The state, quality, or fact of being similar or akin to something else.

Constant

A value that remains unchanged and is not affected by other parameters in a given situation.