Which of the Following Is the Correct Sequence of PCR

question 89

Multiple Choice

Which of the following is the correct sequence of PCR?

• A) Heat to 70°C (to separate DNA), then cool to 50°C (to bind primers and enzymes), and then reheat to 90°C (to synthesize DNA).
• B) Heat to 90°C (to separate DNA), then cool to 50°C (to bind primers and enzymes), and then reheat to 70°C (to synthesize DNA).
• C) Heat to 70°C (to bind primers and enzymes), then cool to 50°C (to synthesize DNA), and then reheat to 90°C (to separate DNA).
• D) Heat to 90°C (to bind primers and enzymes), then cool to 50°C (to separate DNA), and then reheat to 70°C (to synthesize DNA).

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