The PCR (Polymerase Chain Reaction) Protocol That Is Currently Used

question 9

Multiple Choice

The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the discovery of a bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park, in Wyoming. This organism contains a heat -stable form of DNA polymerase known as Taq polymerase, which continues to function even after it has been
Heated to 95°C. Why would such a heat -stable polymerase be beneficial in PCR?

• A) Each cycle includes a "hot" denaturation phase (95°C), which separates the hydrogen bonds that hold the strands of the template DNA together.
• B) Each cycle includes a "hot" denaturation phase (95°C), which serves to sterilize the culture.
• C) Each cycle includes a "hot" saturation phase (95°C), which allows the primers to anneal to the target DNA.
• D) Each cycle includes a "hot" denaturation phase (95°C), which activates the Taq polymerase.
• E) More than one of the above are correct.

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