The PCR (Polymerase Chain Reaction) Protocol That Is Currently Used

question 9

Multiple Choice

The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the discovery of a bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park, in Wyoming. This organism contains a heat -stable form of DNA polymerase known as Taq polymerase, which continues to function even after it has been
Heated to 95°C. Why would such a heat -stable polymerase be beneficial in PCR?

• A) Each cycle includes a "hot" denaturation phase (95°C), which separates the hydrogen bonds that hold the strands of the template DNA together.
• B) Each cycle includes a "hot" denaturation phase (95°C), which serves to sterilize the culture.
• C) Each cycle includes a "hot" saturation phase (95°C), which allows the primers to anneal to the target DNA.
• D) Each cycle includes a "hot" denaturation phase (95°C), which activates the Taq polymerase.
• E) More than one of the above are correct.

Recognize the current definition of menopause and its implications for health sciences.

Understand cultural differences in perceptions and experiences of menopause.

Identify typical endocrine changes during the menopausal transition.

Recognize common symptoms associated with the menopausal transition and their potential connections.

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Definitions:

Median

The middle value in a data set when the numbers are arranged in ascending or descending order, or the mean of the two middle numbers if the data set has an even number of values.

Systolic Blood Pressure

The pressure in the arteries when the heart beats, pushing blood through the blood vessel network.

Normal Model

A probability distribution that is symmetrical around its mean, showing that data near the mean are more frequent in occurrence.

Summary Statistics

Statistical values that provide an overview or summary of a set of data, including measures like mean and standard deviation.