What Is the Most Logical Sequence of Steps for Splicing

question 3

Multiple Choice

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I.Transform bacteria with a recombinant DNA molecule.
II.Cut the plasmid DNA using restriction enzymes (endonucleases) .
III.Extract plasmid DNA from bacterial cells.
IV.Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V.Use ligase to seal plasmid DNA to non-plasmid DNA.

• A) II, III, V, IV, I
• B) III, II, IV, V, I
• C) III, IV, V, I, II
• D) IV, V, I, II, III

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