What Is the Most Logical Sequence of Steps for Splicing

question 3

Multiple Choice

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I.Transform bacteria with a recombinant DNA molecule.
II.Cut the plasmid DNA using restriction enzymes (endonucleases) .
III.Extract plasmid DNA from bacterial cells.
IV.Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V.Use ligase to seal plasmid DNA to non-plasmid DNA.

• A) II, III, V, IV, I
• B) III, II, IV, V, I
• C) III, IV, V, I, II
• D) IV, V, I, II, III

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Definitions:

Socialization

The process by which individuals learn and internalize the norms, values, behaviors, and social skills appropriate to their society.

Single-parent Families

Families headed by only one parent, where the individual takes on the sole responsibility for raising their child or children, due to various circumstances such as divorce, separation, death, or choice.

Industrialized Countries

Nations that are characterized by significant levels of industrial activity, advanced technological infrastructure, and higher standards of living.

Extended Families

A family group that extends beyond the nuclear family to include grandparents, aunts, uncles, and other relatives.