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Nitrogenase, the enzyme that catalyzes nitrogen fixation, is inhibited whenever free oxygen (O₂) reaches a critical concentration. Consequently, nitrogen fixation cannot occur in cells wherein photosynthesis produces free O₂. Consider the colonial aquatic cyanobacterium, Anabaena, whose heterocysts are described as having "...a thickened cell wall that restricts entry of O₂ produced by neighboring cells. Intercellular connections allow heterocysts to transport fixed nitrogen to neighboring cells in exchange for carbohydrates."
Given that the enzymes that catalyze nitrogen fixation are inhibited by oxygen, what mechanism might nitrogen-fixing prokaryotes use to protect these enzymes from oxygen?
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