During Dideoxy Sequencing Reactions,dideoxyribonucleoside Triphosphates DdNTPsare Added in Small Amounts

question 16

Multiple Choice

During dideoxy sequencing reactions,dideoxyribonucleoside triphosphates ddNTPsare added in small amounts to terminate the sequencing reaction.How do ddNTPs stop a sequencing reaction?

• A)ddNTPs lack a 5′ phosphate group.
• B)ddNTPs have a hydroxyl group at their 5′ end.
• C)ddNTPs are irreversible inhibitors on polymerases.
• D)ddNTPs lack a hydroxyl -OHgroup at their 3′ end.

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