Design an Experiment to Demonstrate That Recombination Involved Breakage and Rejoining

question 42

Essay

Design an experiment to demonstrate that recombination involved breakage and rejoining of DNA molecules.E.coli with phage grown in either heavy or light isotopes,and then after allowing for recombination (but not replication),separated the DNA by centrifugation in a CsCl density gradient.The recombinant molecules,consisting of various combinations of heavy or light molecules,would migrate to positions in between the non-recombinant molecules,which consisted entirely of either heavy or light DNA.

---

Definitions:

Business Groups

Collections of businesses, often linked by ownership or control, that work together to achieve economic or political goals.

Racial Balance

Efforts or policies aimed at achieving an equitable representation and integration of various racial groups within institutions or geographic areas, often used in the context of school desegregation and housing.

Supreme Court

The highest judicial body in a country, often having the final appellate jurisdiction and the authority to interpret the constitution, making pivotal decisions on legal and social issues.