You Grow a Barcoded Yeast Deletion Set at 4°C and Then

question 35

Multiple Choice

You grow a barcoded yeast deletion set at 4°C and then a copy of the same set at 25°C.Barcodes from the 4°C sample and 25°C are labeled with a green fluorochrome and a red fluorochrome,respectively.How will you identify genes required for growth specifically at cold temperatures?

• A) separate the PCR products on an agarose gel and look for red fluorescent bands
• B) separate the PCR products on an agarose gel and look for yellow fluorescent bands
• C) hybridize the products to a microarray and look for red spots
• D) hybridize the products to a microarray and look for green spots
• E) separate the PCR products on an agarose gel and look for green fluorescent bands

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Definitions:

Empirical Approach

A method of acquiring knowledge based on observation, experimentation, and evidence rather than theories or pure logic.

Examine Assumptions

The process of critically questioning and evaluating the underlying beliefs or principles that are taken for granted in arguments, theories, or policies.

Observation

The action or process of observing something or someone carefully or in order to gain information.

Evidence-Based Method

A process of making decisions and taking actions based on the best current scientific evidence, typically applied in fields like medicine, psychology, and education.