You Are Conducting a Yeast Two-Hybrid Experiment to Discover Proteins

question 5

Multiple Choice

You are conducting a yeast two-hybrid experiment to discover proteins that interact with a particular protein of interest (protein X) .Which of the following would be a useful step in this process?

• A) Fuse X to the HIS3 promoter.
• B) Fuse X to a DNA binding domain.
• C) Fuse X to the complete HIS3 gene.
• D) Purify X from recombinant yeast.
• E) Fuse X to the complete Gal4 gene.

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Definitions:

MRO

Maintenance, repair, and operating materials.

MRO Inventories

MRO Inventories refer to stocks of Maintenance, Repair, and Operations supplies that are kept on hand to support the production process and facility operations.

ABC Inventory Analysis

A technique that categorizes inventory items into three classes (A, B, and C) based on their importance and value to the organization, to prioritize management and control efforts.

ABC Analysis

is an inventory categorization technique that divides items into three categories (A, B, and C) based on their importance or value, helping to prioritize management and resource allocation.