Which of the Following Limits the Utility of Yeast Two-Hybrid

question 20

Multiple Choice

Which of the following limits the utility of yeast two-hybrid screens in identifying protein interactions?

• A) Yeast two-hybrid screens can only be applied to bait proteins that are less than 20kDa.
• B) Only two proteins can be tested for their ability to interact,per day.
• C) Yeast two-hybrid screens can only find proteins that interact with transcription factors.
• D) Fusion proteins must be affinity purified.
• E) Bait proteins may not fold properly when fused to other proteins.

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Definitions:

Ethnomethodological

Pertaining to the study of the methods people use in everyday life to create a sense of social order and understand their world.

Indifference

A lack of interest, concern, or sympathy towards others or certain situations.

Ethnomethodologists

Researchers who study the methods people use to understand and produce the social order in which they live.

Sociologists

Professionals who study social behavior, institutions, and systems, exploring how humans interact and organize within societies.