You Are Conducting a Yeast Two-Hybrid Experiment to Discover Proteins

question 5

Multiple Choice

You are conducting a yeast two-hybrid experiment to discover proteins that interact with a particular protein of interest (protein X) .Which of the following would be a useful step in this process?

• A) Fuse X to the HIS3 promoter.
• B) Fuse X to a DNA binding domain.
• C) Fuse X to the complete HIS3 gene.
• D) Purify X from recombinant yeast.
• E) Fuse X to the complete Gal4 gene.

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