Which of the Following Limits the Utility of Yeast Two-Hybrid

question 20

Multiple Choice

Which of the following limits the utility of yeast two-hybrid screens in identifying protein interactions?

• A) Yeast two-hybrid screens can only be applied to bait proteins that are less than 20kDa.
• B) Only two proteins can be tested for their ability to interact,per day.
• C) Yeast two-hybrid screens can only find proteins that interact with transcription factors.
• D) Fusion proteins must be affinity purified.
• E) Bait proteins may not fold properly when fused to other proteins.

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