What Is an Advantage of Affinity Purification-Based Methods Over Yeast

question 45

Multiple Choice

What is an advantage of affinity purification-based methods over yeast two-hybrid (Y2H) methods,when screening for interacting proteins?

• A) Affinity methods isolate molecules from their native context and do not require recombinant protein production.
• B) Affinity methods use fusion proteins rather than antibodies,which are more expensive to produce.
• C) Affinity methods,but not Y2H,can be used to find proteins that interact with transcription factors.
• D) Proteins used in affinity methods do not need to be properly folded.
• E) Affinity methods can be applied to proteins from a wider range of eukaryotes than can be used in Y2H.

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