Why Is Trypsin Normally Used in Protein Mass Spectrometry

question 47

Multiple Choice

Why is trypsin normally used in protein mass spectrometry?

• A) It is a positive control found in almost all animal cells.
• B) It is used as a standard to define the upper and lower boundaries of mass sensitivity.
• C) It cuts the protein into smaller peptides,which can be more accurately analyzed than longer fragments.
• D) It is a useful general capture molecule for affinity purification.
• E) It is an effective antibiotic to suppress contamination from microbes.

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Definitions:

Dependent Variable

In scientific research, the variable that is being tested and measured, which is expected to change under the influence of the independent variable.

Independent Variable

In scientific research, the variable that is manipulated or changed to examine its effects on a dependent variable.

International Review Board

A committee that reviews and approves research involving human participants to ensure ethical standards are met.

Ethical Guidelines

Principles designed to govern behavior, often within a professional context, to ensure moral and ethical standards are maintained.