You Label CDNA from a Tumour with a Red Fluorescent

question 22

Multiple Choice

You label cDNA from a tumour with a red fluorescent dye and you use a green dye to label cDNA from normal tissue.Upon microarray hybridization,you find a spot that has more fluorescent signal than the red signal.What is your conclusion?

• A) Increased expression of the gene represented at the green spot is causally related to tumourgenesis.
• B) Transcript of the gene represented at that green spot is less abundant in normal tissue than in the tumour.
• C) Transcripts of all genes are more abundant in normal tissue than in the tumour.
• D) Transcript of the gene represented at that green spot is more abundant in normal tissue than in the tumour.
• E) Decreased expression of the gene represented at the green spot is causally related to tumourgenesis.

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Definitions:

Neutrons

Subatomic particles found in the nucleus of an atom, with no electrical charge and a mass slightly greater than that of protons.

Electron Shells

The different energy states of electrons arranged around the nucleus of an atom.

Atomic Weight

The sum of the weight of the subatomic particles in an average atom of an element, given in atomic mass units.

Green Olivine

A green-colored, magnesium-iron silicate mineral often found in igneous rocks, indicative of certain geological conditions.