Describe How You Would Screen for Arg^- Cells Arising from a Wild-Type

question 75

Essay

Describe how you would screen for Arg^- cells arising from a wild-type culture of E.coli.
To increase frequency of mutation,first expose wild-type culture to a mutagen.Then plate mutagenized cells onto minimal plates containing arginine.After colonies have grown up,replica plate onto minimal plates without arginine.Note position of cells that grow on first set of plates but not the second set.Those are the Arg^- mutants.

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