Describe How You Would Screen for Arg^- Cells Arising from a Wild-Type

question 75

Essay

Describe how you would screen for Arg^- cells arising from a wild-type culture of E.coli.
To increase frequency of mutation,first expose wild-type culture to a mutagen.Then plate mutagenized cells onto minimal plates containing arginine.After colonies have grown up,replica plate onto minimal plates without arginine.Note position of cells that grow on first set of plates but not the second set.Those are the Arg^- mutants.

---

Definitions:

Senior Management

The highest level of executives and managers in an organization who are responsible for its overall operations and strategy.

Implied Repudiation

A situation where one party's actions or statements indicate an intention not to fulfill contractual obligations, thus leading to a breach of contract.

Express Repudiation

The clear and unequivocal refusal to fulfill one's duties or obligations under a contract.

Specific Performance

A legal remedy requiring a party to execute a contract according to its precise terms, often applied in cases where monetary damages are insufficient.