When Bacterial Transformants Containing a Library Are Transferred to Nitrocellulose

question 8

Multiple Choice

When bacterial transformants containing a library are transferred to nitrocellulose filters and screened,one of the steps is treatment with an alkaline solution.What does this do?

• A) attaches the library DNA to the nitrocellulose
• B) promotes hybridization between the probe and complementary inserts
• C) labels the DNA so it can be visualized
• D) stimulates DNA replication,to increase the plasmid copy number
• E) lyses the cells and denatures the released DNA

---

Definitions:

Lymph

A clear fluid that circulates through the lymphatic system, carrying cells that help fight infection and disease.

Bean-Shaped

Descriptive term typically used to describe the shape of organs or structures, notably the kidneys.

Lymphatic Organs

Organs, including the lymph nodes, spleen, and thymus, that are part of the immune system, helping to filter pathogens and produce white blood cells.

Lymph Nodes

Small, bean-shaped structures that are part of the lymphatic system, helping the body fight infections and other diseases.