Genomic DNA Is Isolated from Bacteria

question 21

Multiple Choice

Genomic DNA is isolated from bacteria.After a long digestion with a 6-base cutting enzyme that recognizes the sequence GAATTC,the DNA is not cut.The genome is 4 Mb,so there should be approximately 1,000 sites in the DNA.What is the most likely explanation?

• A) There are no Gs in the genome.
• B) There are no As in the genome.
• C) The bacterium methylated the DNA,because it produces the 6-base cutting enzyme.
• D) All the recognition sites are in genes,and the enzyme will not cut in genes.
• E) All the recognition sites are in promoters and regulatory sequences,and the enzyme cuts only in genes.

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