When Bacterial Transformants Containing a Library Are Transferred to Nitrocellulose

question 8

Multiple Choice

When bacterial transformants containing a library are transferred to nitrocellulose filters and screened,one of the steps is treatment with an alkaline solution.What does this do?

• A) attaches the library DNA to the nitrocellulose
• B) promotes hybridization between the probe and complementary inserts
• C) labels the DNA so it can be visualized
• D) stimulates DNA replication,to increase the plasmid copy number
• E) lyses the cells and denatures the released DNA

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Definitions:

Dementia

Cognitive disorder in which a gradual and usually permanent decline of intellectual functioning occurs; can be caused by a medical condition, substance intoxication, or withdrawal; also known as major neurocognitive disorder.

Substance Intoxication

The physiological state of being under the influence of substances such as drugs or alcohol, affecting mental and physical functions.

Psychological Changes

Alterations in an individual's behavior, feelings, or thoughts that may result from various causes, including stress, trauma, or mental health conditions.

Central Nervous System

The part of the nervous system consisting of the brain and spinal cord, responsible for processing and sending out signals to the body.