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A Phosphate Buffer Solution (25

question 23

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A phosphate buffer solution (25.00 mL sample) used for a growth medium was titrated with 0.1000 M hydrochloric acid. The components of the buffer were sodium monohydrogen phosphate and sodium dihydrogen phosphate. The first endpoint occurred at a volume of 10.32 mL; the second occurred after an additional 18.62 mL was added, for a total volume of 28.94 mL. What was the approximate ratio of HPO42- to H2PO4- in the buffer?

Understand the experimental approaches and findings that have elucidated the roles and mechanisms of plant hormones.
Understand the roles and effects of different plant hormones on growth and development.
Identify the physiological responses of plants to environmental factors and hormonal regulation.
Describe the mechanisms of hormone action in plants including signal transduction.

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