According to the following graph, the activity of the enzyme decreases more sharply at high temperature than at low temperature. What are the reasons for this observation? 
I. As temperature is increased, the substrate binding slowly becomes poorer. II. As temperature is decreased, the enzyme moves more slowly and so reacts more slowly.
III) At higher temperatures, the enzyme is denatured and stops working entirely.
IV) Temperature has no effect on enzyme activity.
Definitions:
Statistical Significance
A measure that indicates how likely a result obtained from a study or experiment is not due to chance.
Sampling Error
Difference between results derived from testing an entire group of events or individuals, and results derived from testing a subset of the group.
Trend Analysis
The process of comparing business data over time to identify any consistent results or trends that can be used to make business decisions or predictions.
Environmental Energy
Energy derived from natural sources in the environment, such as sunlight, wind, and water currents.
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