Assume the Two E

question 5

Multiple Choice

Assume the two E.coli strains shown below are allowed to conjugate. Hfr: pro+, arg+, his+, lys+, met+, ampicillin-sensitive F-: pro-, arg-, his-, lys-, met-, ampicillin-resistant
What supplements would you add to glucose minimal salts agar to select for a recombinant cell that is lys+, arg+, amp-resistant?

• A) ampicillin, proline, histidine, lysine
• B) lysine, arginine
• C) ampicillin, proline, histidine, methionine
• D) proline, histidine, methionine
• E) ampicillin, lysine, arginine

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Definitions:

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