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Table 74
the Results Below Were Obtained from a Use-Dilution Test

question 12

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Table 7.4
The results below were obtained from a use-dilution test of two disinfectants. Cultures were inoculated into tubes with varying concentrations of disinfectants and incubated for 24 hr at 20°C, then subcultured in nutrient media without disinfectants.(+ = growth; - = no growth)
 Disinfectant 1\quad\quad\quad\quad\quad\quad\quad\quad\text { Disinfectant } 1 Disinfectant 2\quad\quad\quad\quad\quad\text { Disinfectant } 2
 Concentration  Initial  Subculture  Initial  Subculture 1:10+1:90++1:900++1:90,000++1:900,000+++1:9,000,000+++\begin{array}{rcccc}\text { Concentration } & \text { Initial } & \text { Subculture } & \text { Initial } & \text { Subculture } \\1: 10 & - & + & - & - \\1: 90 & + & + & - & - \\1: 900 & + & + & - & - \\1: 90,000 & + & + & - & - \\1: 900,000 & + & + & - & + \\1: 9,000,000 & + & + & - & +\end{array} In Table 7.4, what is the minimal bacteriostatic concentration of each disinfectant? Which compound is bactericidal? At what concentration?


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