The Correct Order of Steps to Follow When Using Recombinant

question 41

Multiple Choice

The correct order of steps to follow when using recombinant DNA technology to produce a protein for pharmacological use is

• A) isolate the desired gene - purify the expressed protein - grow the transformed cells into suitable quantities - insert the gene into an expression vector - transform host cells with the expression vector.
• B) purify the expressed protein - insert the gene into an expression vector - transform host cells with the expression vector - grow the transformed cells into suitable quantities - isolate the desired gene.
• C) grow the transformed cells into suitable quantities - transform host cells with the expression vector - insert the gene into an expression vector -isolate the desired gene - purify the expressed protein.
• D) transform host cells with the expression vector - grow the transformed cells into suitable quantities - isolate the desired gene - insert the gene into an expression vector - purify the expressed protein.
• E) isolate the desired gene - insert the gene into an expression vector - transform host cells with the expression vector - grow the transformed cells into suitable quantities - purify the expressed protein.

Recognize the role of association areas in integrating sensory inputs and directing motor outputs.

Understand the roles and functions of the nervous system.

Comprehend the basics of neurochemistry and the role of neurotransmitters.

Identify the components and functions of the central and peripheral nervous systems.

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Definitions:

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The revocation or denial of voting rights, often used historically to exclude specific groups from political participation based on race, gender, or socio-economic status.