The Correct Order of Steps to Follow When Using Recombinant

question 41

Multiple Choice

The correct order of steps to follow when using recombinant DNA technology to produce a protein for pharmacological use is

• A) isolate the desired gene - purify the expressed protein - grow the transformed cells into suitable quantities - insert the gene into an expression vector - transform host cells with the expression vector.
• B) purify the expressed protein - insert the gene into an expression vector - transform host cells with the expression vector - grow the transformed cells into suitable quantities - isolate the desired gene.
• C) grow the transformed cells into suitable quantities - transform host cells with the expression vector - insert the gene into an expression vector -isolate the desired gene - purify the expressed protein.
• D) transform host cells with the expression vector - grow the transformed cells into suitable quantities - isolate the desired gene - insert the gene into an expression vector - purify the expressed protein.
• E) isolate the desired gene - insert the gene into an expression vector - transform host cells with the expression vector - grow the transformed cells into suitable quantities - purify the expressed protein.

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