Put the Following Steps of the Production of a Recombinant

question 53

Multiple Choice

Put the following steps of the production of a recombinant vector, containing a human gene, in order:
I. Introduce the recombinant plasmid into a bacterial cell.
II. Isolate the human gene and the vector DNA using restriction enzymes.
III. Ligate the DNA fragments to produce a recombinant plasmid.
IV. Grow bacterial cells on a medium containing a selective agent (such as an antibiotic) .

• A) III, I, IV, II
• B) I, III, IV, II
• C) II, I, III, IV
• D) II, III, I, IV
• E) III, I, II, IV

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