If Single- Stranded Binding Protein (SSB) Is NOT Present During

question 36

Multiple Choice

If Single- Stranded Binding protein (SSB) is NOT present during DNA replication, what would you expect to see?

• A) The replisome complex would not assemble on the oriC region.
• B) SSB carries the helicase protein to the open region of DNA, so hydrolysis and strand separation will not occur.
• C) Helicase activity is inhibited, so DNA strands cannot be separated.
• D) SSB prevents reannealing of the separated strands, so strands would quickly reanneal and DNA replication cannot proceed.
• E) The DNA cannot bend, so hydrogen bonds in the 13- mer region of oriC remain intact.

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