To Map Genes of a Bacterial Strain, Conjugation Must Be

question 9

Multiple Choice

To map genes of a bacterial strain, conjugation must be interrupted at given times. Suppose you have Hfr cells of genotype a+b+c+d+e+strR and F- cells of genotype a- b- c- d- e- strS and you combine these two cultures in liquid medium in four blenders at time 0. After intervals of 3, 6, 9, and 12 minutes, you turn on successive blenders. The graph below shows the results of plating the cultures from each blender. What is the correct order of the genes on this chromosome?

• A) a b c d e
• B) b e c a d
• C) a c e b d
• D) d a c e b
• E) b e c a (d is unknown)

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