You Have a Linear DNA Fragment of 1600 Bp

question 5

Multiple Choice

You have a linear DNA fragment of 1600 bp. To characterize it, you decide to make a restriction map. Cutting with one enzyme, you show by electrophoresis that the fragments produced are 400 and 1200 bp. Cutting the same DNA with another enzyme you recover 700 and 900 bp fragments. After treatment with both enzymes, fragments were 300, 400, and 900 bp. Which of the following order of fragments, if any, best represents the restriction map?

• A) 400/ 300/ 900
• B) 900/ 400/ 300
• C) 400/ 900/ 300
• D) 300/ 400/ 900
• E) It is not possible to determine the order.

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