Efficient Production of Eukaryotic Proteins in E

question 22

Multiple Choice

Efficient production of eukaryotic proteins in E. coli may require all of the following except?

• A) altered codon usage within the heterologous sequences to approximate the codon bias in E. coli
• B) the use of cDNAs, that are free of introns, as eukaryotic transgenes
• C) an E. coli expression vector with a promoter sequence that binds RNA polymerase
• D) an E. coli expression vector with a Shine- Dalgarno sequence for efficient translation
• E) a negative selectable marker to select against nonhomologous recombination events

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